Gal3醛固酮是预测肺动脉高压的潜在
摘要
背景：很多研究证实和肺动脉高压（PAH）的发生和严重程度与血清标志物相关。近期研究证实Gal-3-醛固酮在左心衰竭中的作用。然而，Gal-3联合醛固酮在PAH中的作用尚未报道。
目的：本研究旨在探讨Gal-3-醛固酮模式及其他潜在的PAH生物标志物及其与PAH严重程度的关系。
方法：本研究总共纳入57例患者，其中41例是特发性肺动脉高压(IPAH)。IPAH和16例CTD相关PAH,对照组为8例年龄相匹配的非PAH者。Gal-3,醛固酮和其他血清标志物使用ELISA等方法检测。
结果：Gal-3水平在IPAH(12.2±0.6ng/mL;p0.05)和PAH-CTD(14.1±1.6ng/mL;p0.05)组较对照组(8.5±0.9ng/mL)显著升高，同时，醛固酮水平仅在IPAH组(.5±38.8pg/mLvscontrol71.9±18.2pg/mL;p0.05)升高。此外，WHO心功能II–III级与I级或对照组相比，Gal-3和NT-proBNP水平明显升高。IPAH和PAH-CTD与对照组比较ICAM-1水平明显升高(.5±18.2pg/mLand.1±59.4pg/mLvscontrols.8±39.3pg/mL,p0.05,p0.,)。然而，VCAM-1和前炎细胞，及IL-12仅在PAH-CTD组升高(.5±.0pg/mLand.2±70.3pg/mLvscontrols.8±44.6pg/mL,p0.01,and.1±15.2pg/mL,p0.01,)。
结论：PAH患者Gal-3和醛固酮血清学水平升高证实了Gal-3信号通路在IPAH和PAH-CTD机制中的作用，可能是预测疾病进展和功能状态的标志物。
Galectin-3andaldosteroneaspotentialtandembiomarkersinpulmonaryarterialhypertensionLaurentCalvier,EkaterinaLegchenko,LenaGrimm,HannesSallmon,AdamHatch,BrianDPlouffe,ChristophSchroeder,4JohannBauersachs,ShashiKMurthy,GeorgHansmannAbstract
Background
Severalstudieshaveidentifiedcirculatingbiomarkerstobeassociatedwiththepresenceandseverityofpulmonaryarterialhypertension(PAH).Recentevidencesupportsaroleforgalectin-3(Gal-3)andthemineralcorticoidaldosteroneinleftventricularfailure.However,studiesonaldosteronetogetherwithGal-3inPAHarelacking.
Objective
WeinvestigatedanovelAldosterone-galectin3(Gal-3)tandemandseveralotherpotentialPAHbiomarkersandtheirassociationwiththediseaseseverity.
Methods
Atotalof57patients,41withidiopathicPAH.(IPAH)and16withPAHassociatedwithconnectivetissuedisease(CTD),and8age-matched,non-relativecontrolswerestudied.Gal-3,aldosteroneandotherpotentialproteinplasmaconcentrationsweremeasuredbysingleELISAandmulti-arrayMSD(MesoScaleDiscovery)technology.
Results
Gal-3valueswereincreasedinbothpatientswithIPAH(12.2±0.6ng/mL;p0.05)andwithPAH-CTD(14.1±1.6ng/mL;p0.05)versuscontrol(8.5±0.9ng/mL),whilealdosteronewassignificantlyelevatedinIPAHonly(.5±38.8pg/mLvscontrol71.9±18.2pg/mL;p0.05).Inaddition,aldosterone,Gal-3,andN-terminalpro-brainnatriureticpeptide(NT-proBNP)valueswereallhigherinpatientsinWHOfunctionalclassII–IIIversusPAHfunctionalclassIorcontrols.Thevascularinjurymarkerintercellularadhesionmolecule1(ICAM-1)wasincreasedinIPAHandPAH-CTDversuscontrols(.5±18.2pg/mLand.1±59.4pg/mLvscontrols.8±39.3pg/mL,p0.05,p0.,respectively),whereasvascularcelladhesionmolecule1(VCAM-1)andproinflammatory,anti-angiogenicinterleukin-12(IL-12)wereelevatedinPAH-CTDonly(.5±.0pg/mLand.2±70.3pg/mLvscontrols.8±44.6pg/mL,p0.01,and.1±15.2pg/mL,p0.01,respectively).
Conclusions
HeightenedGal-3andaldosteroneplasmaconcentrationsinPAHpatientsindicatearoleforGal-3signallinginthepathobiologyofIPAHandPAH-CTD,andmayserveasbiomarkersforfunctionalstatusandprogressionofdisease.
Heart;:–.doi:10./heartjnl--
编译者：医院临床免疫科韩青
